Sex determination using circulating cell-free fetal DNA in small volume of maternal plasma in elephants

Prenatal determination of farm animal fetal sex using free fetal DNA in maternal plasma

In many farm animal species, the preference of a specific sex is obvious mainly due to commercial implications; so for comprehensive and efficient results in the livestock management plans specially breedingand selection programs, it is necessary to include acost-effective and reliable strategy for early determination of fetal sex. Several invasive and non-invasive approaches have been establis...

Evaluation of Sample Stability and Automated DNA Extraction for Fetal Sex Determination Using Cell-Free Fetal DNA in Maternal Plasma

OBJECTIVE The detection of paternally inherited sequences in maternal plasma, such as the SRY gene for fetal sexing or RHD for fetal blood group genotyping, is becoming part of daily routine in diagnostic laboratories. Due to the low percentage of fetal DNA, it is crucial to ensure sample stability and the efficiency of DNA extraction. We evaluated blood stability at 4°C for at least 24 hours a...

fetal sex determination using non-invasive method of cell-free fetal dna in maternal plasma of pregnant women during 6th– 10th weeks of gestation

in previous years, identification of fetal cells in maternal blood circulation has caused a new revolution in non-invasive method of prenatal diagnosis. low number of fetal cells in maternal blood and long-term survival after pregnancy limited the use of fetal cells in diagnostic and clinical applications. with the discovery of cell-free fetal dna (cffdna) in plasma of pregnant women, access to...

A Low-Cost Efficient Multiplex P CR for Prenatal Sex Determination in Bovine Fetus U sing Free Fetal DNA in Maternal Plasma

early non-invasive determination of fetal sex using cell-free dna

the first step in the prenatal diagnosis of x-linked genetic disorders is determining fetus gender. current invasive methods to obtain the dna source of the fetus instead of its miscarriage risk, has harmful stress for high risk pregnancies. cell free fetal dna (cffdna) circulating in the maternal blood, has now become a useful source of noninvasive prenatal diagnosis. considering limitation of...